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Evidence of specific characteristics and osteogenic potentiality in bone cells from tibia
Journal of cellular physiology, 2011-10, Vol.226 (10), p.2675-2682
Manferdini, Cristina
Gabusi, Elena
Grassi, Francesco
Piacentini, Anna
Cattini, Luca
Zini, Nicoletta
Filardo, Giuseppe
Facchini, Andrea
Lisignoli, Gina
2011
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Manferdini, Cristina
Gabusi, Elena
Grassi, Francesco
Piacentini, Anna
Cattini, Luca
Zini, Nicoletta
Filardo, Giuseppe
Facchini, Andrea
Lisignoli, Gina
Titel
Evidence of specific characteristics and osteogenic potentiality in bone cells from tibia
Ist Teil von
Journal of cellular physiology, 2011-10, Vol.226 (10), p.2675-2682
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2011
Quelle
MEDLINE
Beschreibungen/Notizen
Human bone cells used for in vitro studies are mainly derived from bone marrow (BM) or trabecular bone (TB). There are no specific markers or procedures for isolation and growth of these cells. To validate the potentiality of these cells, we isolated human mesenchymal stromal cells (MSCs) and osteoblasts (OBs) from the tibial plateau of the same subject, grown in two different media (α‐MEM and DMEM/F12) and analyzed for cell growth, proliferation, phenotype and osteogenic potential. We found that OBs grew well in both media tested, but MSCs were able to grow only in α‐MEM medium. OBs in DMEM/F12 showed reduced proliferation capability and expressed a low level of alkaline phosphatase (AP), RUNX‐2, osteocalcin (OC), bone sialoprotein (BSP), collagen type I (Col.I) compared with OBs in α‐MEM but high level of collagen type XV (Col.XV). Compared with MSCs in α‐MEM, OBs have an increased ability to proliferate and express more OC and BSP at molecular level but less AP, RUNX‐2 and Col.I than MSCs. Time‐course experiments to analyze the osteogenic potential of these cells showed that OBs were more efficient than MSCs. However, these cells obtained from tibial plateau showed a different trend of AP, OC and Col.I osteogenic markers compared to control MSCs from the iliac crest. This study shows that bone‐adherent OBs grown in α‐MEM medium are more efficient for osteogenic differentiation than BM MSCs and contribute to defining their phenotypic and functional characteristics, so providing a rationale for their use in bone tissue engineering or therapeutic purposes. J. Cell. Physiol. 226: 2675–2682, 2011. © 2011 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0021-9541
eISSN: 1097-4652
DOI: 10.1002/jcp.22618
Titel-ID: cdi_proquest_miscellaneous_879679556
Format
–
Schlagworte
Aged
,
Bone Marrow Cells - cytology
,
Bone Marrow Cells - physiology
,
Cell Separation - methods
,
Cells, Cultured
,
Humans
,
Middle Aged
,
Osteoarthritis, Knee - surgery
,
Osteoblasts - cytology
,
Osteoblasts - physiology
,
Osteogenesis - genetics
,
Osteogenesis - physiology
,
Stem Cells - cytology
,
Stem Cells - physiology
,
Stromal Cells - cytology
,
Stromal Cells - physiology
,
Tibia - cytology
,
Tissue and Organ Harvesting - methods
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