Details

Autor(en) / Beteiligte

• Pan, Min-Hui
• Cai, Xiu-Juan
• Liu, Min
• Lv, Jun
• Tang, Hui
• Tan, Juan
• Lu, Cheng

Titel

Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori

Ist Teil von

• Tissue & cell, 2010-02, Vol.42 (1), p.42-46

Ort / Verlag

Scotland: Elsevier Ltd

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2 n = 56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7 h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID50 ) at a cell density of 105 cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line.