Biological chemistry, 2011-04, Vol.392 (4), p.327-336
2011
Details
- Autor(en) / Beteiligte
- Titel
- The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines
- Ist Teil von
- Biological chemistry, 2011-04, Vol.392 (4), p.327-336
- Ort / Verlag
- Germany: Walter de Gruyter
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Supplementary to the efficient inhibition of trypsin, chymotrypsin, plasma kallikrein, and plasmin already described by the EcTI inhibitor from Enterolobium contortisiliquum, it also blocks human neutrophil elastase (K iapp=4.3 nm) and prevents phorbol ester (PMA)-stimulated activation of matrix metalloproteinase (MMP)-2 probably via interference with membrane-type 1 (MT1)-MMP. Moreover, plasminogen-induced activation of proMMP-9 and processing of active MMP-2 was also inhibited. Furthermore, the effect of EcTI on the human cancer cell lines HCT116 and HT29 (colorectal), SkBr-3 and MCF-7 (breast), K562 and THP-1 (leukemia), as well as on human primary fibroblasts and human mesenchymal stem cells (hMSCs) was studied. EcTI inhibited in a concentration range of 1.0–2.5 μm rather specifically tumor cell viability without targeting primary fibroblasts and hMSCs. Taken together, our data indicate that the polyspecific proteinase inhibitor EcTI prevents proMMP activation and is cytotoxic against tumor cells without affecting normal tissue remodeling fibroblasts or regenerative hMSCs being an important tool in the studies of tumor cell development and dissemination.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1431-6730eISSN: 1437-4315DOI: 10.1515/bc.2011.031Titel-ID: cdi_proquest_miscellaneous_879102454
- Format
- –
- Schlagworte
- Breast Neoplasms - drug therapy, cancer, Cell Line, Tumor, Cell Survival - drug effects, Colorectal Neoplasms - drug therapy, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Fabaceae - chemistry, Female, Fibroblasts - drug effects, Humans, Kunitz inhibitors, Leukemia - drug therapy, Leukocyte Elastase - antagonists & inhibitors, Matrix Metalloproteinase 14 - metabolism, Matrix Metalloproteinase 2 - metabolism, Matrix Metalloproteinase 9 - metabolism, matrix metalloproteinases, mesenchymal stem cells, Mesenchymal Stromal Cells - drug effects, plant proteinases, Plant Proteins - pharmacology, Plasminogen - pharmacology, Protease Inhibitors - pharmacology, Tetradecanoylphorbol Acetate - pharmacology, Trypsin Inhibitors - pharmacology