Archives of virology, 2011-06, Vol.156 (6), p.931-938
2011
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Details
- Autor(en) / Beteiligte
- Titel
- The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus
- Ist Teil von
- Archives of virology, 2011-06, Vol.156 (6), p.931-938
- Ort / Verlag
- Vienna: Springer Vienna
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0304-8608eISSN: 1432-8798DOI: 10.1007/s00705-011-0927-xTitel-ID: cdi_proquest_miscellaneous_876236713
- Format
- –
- Schlagworte
- Amino Acid Sequence, Amino acids, Animals, Binding Sites, Biological and medical sciences, Biomedical and Life Sciences, Biomedicine, Cercopithecus aethiops, Cloning, Conserved Sequence, Dengue fever, Encephalitis, Flavivirus, Fundamental and applied biological sciences. Psychology, HeLa Cells, Humans, Immunoprecipitation, Infectious Diseases, Medical Microbiology, Microbiology, Miscellaneous, Original Article, Pathogenesis, Plasmids, Polymerase Chain Reaction, Polypeptides, Protein Binding, Protein Interaction Domains and Motifs, Proteins, Ribonucleoprotein, U1 Small Nuclear - chemistry, Ribonucleoprotein, U1 Small Nuclear - metabolism, RNA polymerase, RNA, Viral, Two-Hybrid System Techniques, Vaccines, Vero Cells, Viral Nonstructural Proteins - chemistry, Viral Nonstructural Proteins - metabolism, Virology, Viruses, West Nile virus, Yellow fever virus, Yellow fever virus - genetics, Yellow fever virus - metabolism