Details

Autor(en) / Beteiligte

• Bar-On, Liat
• Birnberg, Tal
• Lewis, Kanako L
• Edelson, Brian T
• Bruder, Dunja
• Hildner, Kai
• Buer, Jan
• Murphy, Kenneth M
• Reizis, Boris
• Jung, Steffen

Titel

CX3CR1+ CD8a+ dendritic cells are a steady-state population related to plasmacytoid dendritic cells

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2010-08, Vol.107 (33), p.14745-14750

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Lymphoid organs are characterized by a complex network of phenotypically distinct dendritic cells (DC) with potentially unique roles in pathogen recognition and immunostimulation. Classical DC (cDC) include two major subsets distinguished in the mouse by the expression of CD8a. Here we describe a subset of CD8a super(+) DC in lymphoid organs of naive mice characterized by expression of the CX sub(3)CR1 chemokine receptor. CX sub(3)CR1 super(+) CD8a super(+) DC lack hallmarks of classical CD8a super(+) DC, including IL-12 secretion, the capacity to cross-present antigen, and their developmental dependence on the transcriptional factor BatF3. Gene-expression profiling showed that CX sub(3)CR1 super(+) CD8a super(+) DC resemble CD8a super(-) cDC. The microarray analysis further revealed a unique plasmacytoid DC (PDC) gene signature of CX sub(3)CR1 super(+) CD8a super(+) DC. A PDC relationship of the cells is supported further by the fact that they harbor characteristic D-J Ig gene rearrangements and that development of CX sub(3)CR1 super(+) CD8a super(+) DC requires E2-2, the critical transcriptional regulator of PDC. Thus, CX sub(3)CR1 super(+) CD8a super(+) DC represent a unique DC subset, related to but distinct from PDC. Collectively, the expression-profiling data of this study refine the resolution of previous DC definitions, sharpen the border of classical CD8a super(+) and CD8a super(-) DC, and should assist the identification of human counterparts of murine DC subsets.