Bioscience, biotechnology, and biochemistry, 2011, Vol.75 (6), p.1119-1128
2011
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- Autor(en) / Beteiligte
- Titel
- A Novel Small Protein of Bacillus subtilis Involved in Spore Germination and Spore Coat Assembly
- Ist Teil von
- Bioscience, biotechnology, and biochemistry, 2011, Vol.75 (6), p.1119-1128
- Ort / Verlag
- Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Two small genes named sscA (previously yhzE) and orf-62, located in the prsA-yhaK intergenic region of the Bacillus subtilis genome, were transcribed by SigK and GerE in the mother cells during the later stages of sporulation. The SscA-FLAG fusion protein was produced from T 5 of sporulation and incorporated into mature spores. sscA mutant spores exhibited poor germination, and Tricine-SDS-PAGE analysis showed that the coat protein profile of the mutant differed from that of the wild type. Bands corresponding to proteins at 59, 36, 5, and 3 kDa were reduced in the sscA null mutant. Western blot analysis of anti-CotB and anti-CotG antibodies showed reductions of the proteins at 59 kDa and 36 kDa in the sscA mutant spores. These proteins correspond to CotB and CotG. By immunoblot analysis of an anti-CotH antibody, we also observed that CotH was markedly reduced in the sscA mutant spores. It appears that SscA is a novel spore protein involved in the assembly of several components of the spore coat, including CotB, CotG, and CotH, and is associated with spore germination.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0916-8451eISSN: 1347-6947DOI: 10.1271/bbb.110029Titel-ID: cdi_proquest_miscellaneous_873705176
- Format
- –
- Schlagworte
- Amino Acid Sequence, Bacillus subtilis, Bacillus subtilis - genetics, Bacillus subtilis - metabolism, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Biological and medical sciences, Blotting, Western, DNA, Intergenic - chemistry, DNA, Intergenic - genetics, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Bacterial, germination, Molecular Sequence Data, Mutation, Plasmids, Polymerase Chain Reaction, Promoter Regions, Genetic, Recombinant Fusion Proteins - genetics, Recombinant Fusion Proteins - metabolism, RNA, Messenger - analysis, RNA, Messenger - biosynthesis, small protein, spore coat, Spores, Bacterial - chemistry, Spores, Bacterial - genetics, Spores, Bacterial - metabolism, sscA, Transcription, Genetic, Transformation, Bacterial