Journal of insect physiology, 2010-09, Vol.56 (9), p.1087-1094
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Triazophos up-regulated gene expression in the female brown planthopper, Nilaparvata lugens
- Ist Teil von
- Journal of insect physiology, 2010-09, Vol.56 (9), p.1087-1094
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The widespread use of insecticides has caused the resurgence of the brown planthopper, Nilaparvata lugens, in Asia. In this study, we investigated an organo-phosphorous insecticide, triazophos, and its ability to induce gene expression variation in female N. lugens nymphs just before emergence. By using the suppression subtractive hybridization method, a triazophos-induced cDNA library was constructed. In total, 402 differentially expressed cDNA clones were obtained. Real-time qPCR analysis confirmed that triazophos up-regulated the expression of six candidate genes at the transcript level in nymphs on day 3 of the 5th instar. These genes encode N. lugens vitellogenin, bystin, multidrug resistance protein (MRP), purine nucleoside phosphorylase (PNP), pyrroline-5-carboxylate reductase (P5CR) and carboxylesterase. Our results imply that the up-regulation of these genes may be involved in the induction of N. lugens female reproduction or resistance to insecticides.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1910eISSN: 1879-1611DOI: 10.1016/j.jinsphys.2010.03.004Titel-ID: cdi_proquest_miscellaneous_872124527
- Format
- –
- Schlagworte
- Animals, ATP Binding Cassette Transporter, Sub-Family B - metabolism, Carboxylesterase, Cell Adhesion Molecules - metabolism, complementary DNA, DNA Primers - genetics, Female, females, gene expression, gene expression regulation, Gene Expression Regulation - drug effects, Gene Library, Hemiptera - drug effects, Hemiptera - metabolism, insect pests, insect proteins, insect reproduction, insecticide resistance, Insecticides - toxicity, messenger RNA, Nilaparvata lugens, nucleic acid hybridization, Nymph - drug effects, Nymph - metabolism, nymphs, Organothiophosphates - toxicity, plant pests, Polymerase Chain Reaction, Purine-Nucleoside Phosphorylase - metabolism, Pyrroline Carboxylate Reductases - metabolism, Resurgence, Suppression subtractive hybridization, Triazoles - toxicity, Triazophos, Vitellogenins - metabolism