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A simulated aerobic composting experiment was used to explore the effects of sulphur and Thiobacillus thioparus during six manure composting treatments. The addition of sulphur led to a decrease of the pH level within the range 6–6.3, which was lower than the control treatment (CK). The concentration of ammonium nitrogen in T1 (0.25% sulphur), T2 (0.5% sulphur), T3 (0.25% sulphur+T. thioparus) and T4 (0.5% sulphur+T. thioparus) were much higher than the ammonium N in CK. The results indicated that addition of sulphur could increase the concentration of ammonium N and reduce loss of nitrogen. However, excess sulphur had a negative effect on temperature and GI. Addition of T. thioparus could increase concentration of available S, alleviate these negative influences and reduce compost biological toxicity.