Zhong hua yi xue za zhi, 2011-03, Vol.91 (10), p.694-698
2011
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- Autor(en) / Beteiligte
- Titel
- Rapamycin-mediated interaction of spleen γδT lymphocytes and lung macrophages in acute lung injury of mice induced by lipopolysaccharide
- Ist Teil von
- Zhong hua yi xue za zhi, 2011-03, Vol.91 (10), p.694-698
- Ort / Verlag
- China
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To explore the influences of rapamycin (RAPA) upon the cytokine changes of activated spleen γδT lymphocytes and lung tissue macrophages in acute lung injury of mice induced by lipopolysaccharide (LPS). A total of 24 healthy male C57BL/6 mice, 6-8 weeks old, were randomly divided into phosphate buffered saline (PBS), LPS, RAPA and LPS + RAPA groups. Acute lung injury was induced by a single intratracheal instillation of LPS in mice. And spleen γδT lymphocytes and lung macrophages were purified by immunomagnetic beads at Day 1. The purified spleen γδT lymphocytes and lung macrophages were adjusted to 10(6) cell/ml. And 24-well plates were used for 4 groups. Each group were further separated with spleen γδT lymphocytes alone, lung tissue macrophages alone and co-culturing. Supernatant fluid was collected after 24 hours. The expressions of IFN-γ and TNF-α were analyzed by ELISA (enzyme-linked immunosorbent assay). And the expressions of mRNA were analyzed by real-time quantitative PCR (polymerase chain reaction).
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0376-2491Titel-ID: cdi_proquest_miscellaneous_868769584
- Format
- –
- Schlagworte
- Acute Lung Injury - metabolism, Animals, Cell Communication, Coculture Techniques, Interferon-gamma - secretion, Lipopolysaccharides, Macrophages, Alveolar - cytology, Macrophages, Alveolar - drug effects, Male, Mice, Mice, Inbred C57BL, Sirolimus - pharmacology, Spleen - cytology, Spleen - drug effects, T-Lymphocytes - cytology, T-Lymphocytes - drug effects, Tumor Necrosis Factor-alpha - secretion