Biochemistry (Moscow), 2011-04, Vol.76 (4), p.455-461
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Heat shock protein DnaK — Substrate of actin-specific bacterial protease ECP32
- Ist Teil von
- Biochemistry (Moscow), 2011-04, Vol.76 (4), p.455-461
- Ort / Verlag
- Dordrecht: SP MAIK Nauka/Interperiodica
- Erscheinungsjahr
- 2011
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- It has been found that actin-specific bacterial protease ECP32 cleaves prokaryotic heat shock protein DnaK, which belongs to the family of heat shock proteins with molecular weight 70 kDa. We propose a new one-step method for DnaK purification using heat treatment. The technique yields ∼1 mg of partially purified DnaK from 25 g of wet bacterial biomass. Polyclonal antibodies against DnaK were obtained. The degree of ECP32 catalyzed proteolysis of partially purified DnaK and that of DnaK in initial cell extracts was compared.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-2979eISSN: 1608-3040DOI: 10.1134/S0006297911040080Titel-ID: cdi_proquest_miscellaneous_867725654
- Format
- –
- Schlagworte
- Actin, Actins - chemistry, Antibodies, Bacillus subtilis - enzymology, Bacteria, Biochemistry, Biomedical and Life Sciences, Biomedicine, Bioorganic Chemistry, Candida albicans - enzymology, Endopeptidases - chemistry, Escherichia coli - enzymology, Escherichia coli Proteins - chemistry, Escherichia coli Proteins - immunology, Escherichia coli Proteins - isolation & purification, Gram-Negative Bacteria - enzymology, Heat shock proteins, HSP70 Heat-Shock Proteins - chemistry, HSP70 Heat-Shock Proteins - immunology, HSP70 Heat-Shock Proteins - isolation & purification, Immune Sera, Life Sciences, Microbiology, Muscle proteins, Peptides, Proteases, Proteolysis, Saccharomyces cerevisiae - enzymology, Sequence Analysis, Protein, Substrates, Viral antibodies