Details

Autor(en) / Beteiligte

• SMITH, B. L.
• LU, C.-P.
• ALVARADO BREMER, J. R.

Titel

High-resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies

Ist Teil von

• Molecular ecology resources, 2010-01, Vol.10 (1), p.193-196

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• High‐resolution melting analysis (HRMA) is a highly sensitive closed‐tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase‐A (ldh‐A), myosin light chain‐2 (mlc‐2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.