Molecular cell, 2011-05, Vol.42 (3), p.330-341
- Schmitges, Frank W.
- Prusty, Archana B.
- Faty, Mahamadou
- Stützer, Alexandra
- Lingaraju, Gondichatnahalli M.
- Aiwazian, Jonathan
- Sack, Ragna
- Hess, Daniel
- Li, Ling
- Zhou, Shaolian
- Bunker, Richard D.
- Wirth, Urs
- Bouwmeester, Tewis
- Bauer, Andreas
- Ly-Hartig, Nga
- Zhao, Kehao
- Chan, Homan
- Gu, Justin
- Gut, Heinz
- Fischle, Wolfgang
- Müller, Jürg
- Thomä, Nicolas H.
2011
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- Autor(en) / Beteiligte
- Schmitges, Frank W.
- Prusty, Archana B.
- Faty, Mahamadou
- Stützer, Alexandra
- Lingaraju, Gondichatnahalli M.
- Aiwazian, Jonathan
- Sack, Ragna
- Hess, Daniel
- Li, Ling
- Zhou, Shaolian
- Bunker, Richard D.
- Wirth, Urs
- Bouwmeester, Tewis
- Bauer, Andreas
- Ly-Hartig, Nga
- Zhao, Kehao
- Chan, Homan
- Gu, Justin
- Gut, Heinz
- Fischle, Wolfgang
- Müller, Jürg
- Thomä, Nicolas H.
- Titel
- Histone Methylation by PRC2 Is Inhibited by Active Chromatin Marks
- Ist Teil von
- Molecular cell, 2011-05, Vol.42 (3), p.330-341
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The Polycomb repressive complex 2 (PRC2) confers transcriptional repression through histone H3 lysine 27 trimethylation (H3K27me3). Here, we examined how PRC2 is modulated by histone modifications associated with transcriptionally active chromatin. We provide the molecular basis of histone H3 N terminus recognition by the PRC2 Nurf55-Su(z)12 submodule. Binding of H3 is lost if lysine 4 in H3 is trimethylated. We find that H3K4me3 inhibits PRC2 activity in an allosteric fashion assisted by the Su(z)12 C terminus. In addition to H3K4me3, PRC2 is inhibited by H3K36me2/3 (i.e., both H3K36me2 and H3K36me3). Direct PRC2 inhibition by H3K4me3 and H3K36me2/3 active marks is conserved in humans, mouse, and fly, rendering transcriptionally active chromatin refractory to PRC2 H3K27 trimethylation. While inhibition is present in plant PRC2, it can be modulated through exchange of the Su(z)12 subunit. Inhibition by active chromatin marks, coupled to stimulation by transcriptionally repressive H3K27me3, enables PRC2 to autonomously template repressive H3K27me3 without overwriting active chromatin domains. [Display omitted] ► The PRC2 Nurf55-Su(z)12 subcomplex binds the N-terminal tail of histone H3 ► H3K4me3-containing tails are no longer recognized by Nurf55-Su(z)12 ► H3K4me3 and H3K36me3 tails trigger direct allosteric inhibition of PRC2 ► Transcriptionally active chromatin inhibits deposition of repressive marks by PRC2
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2011.03.025Titel-ID: cdi_proquest_miscellaneous_865689669
- Format
- –
- Schlagworte
- Allosteric properties, Amino Acid Sequence, Animals, Blotting, Western, Cell Line, chromatin, Chromatin - genetics, Chromatin - metabolism, Crystallography, X-Ray, Drosophila, Drosophila Proteins - chemistry, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Histone-Lysine N-Methyltransferase - chemistry, Histone-Lysine N-Methyltransferase - genetics, Histone-Lysine N-Methyltransferase - metabolism, histones, Histones - chemistry, Histones - metabolism, Humans, lysine, Lysine - chemistry, Lysine - metabolism, Methylation, Mice, Models, Molecular, Molecular Sequence Data, Mutation, Polycomb Repressive Complex 2, Polycomb-Group Proteins, Protein Binding, Protein Structure, Tertiary, Repressor Proteins - chemistry, Repressor Proteins - genetics, Repressor Proteins - metabolism, Retinoblastoma-Binding Protein 4 - chemistry, Retinoblastoma-Binding Protein 4 - genetics, Retinoblastoma-Binding Protein 4 - metabolism, transcription (genetics), Transcription, Genetic