Virology (New York, N.Y.), 2011-05, Vol.413 (2), p.283-292
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Antiviral activity of bovine type III interferon against foot-and-mouth disease virus
- Ist Teil von
- Virology (New York, N.Y.), 2011-05, Vol.413 (2), p.283-292
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2011
- Quelle
- Elektronische Zeitschriftenbibliothek
- Beschreibungen/Notizen
- Foot-and-mouth disease (FMD) is one of the most serious threats to the livestock industry. Despite the availability of a vaccine, recent outbreaks in disease-free countries have demonstrated that development of novel FMD control strategies is imperative. Here we report the identification and characterization of bovine (bo) interferon lambda 3 (IFN-λ3), a member of the type III IFN family. Expression of boIFN-λ3 using a replication-defective human adenovirus type 5 vector (Ad5-boIFN-λ3) yielded a glycosylated secreted protein with antiviral activity against FMD virus (FMDV) and vesicular stomatitis virus in bovine cell culture. Inoculation of cattle with Ad5-boIFN-λ3 induced systemic antiviral activity and up-regulation of IFN stimulated gene expression in multiple tissues susceptible to FMDV infection. Our results demonstrate that the type III IFN family is conserved in bovines and boIFN-λ3 has potential for further development as a biotherapeutic candidate to inhibit FMDV or other viruses in cattle.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0042-6822, 1096-0341eISSN: 1096-0341DOI: 10.1016/j.virol.2011.02.023Titel-ID: cdi_proquest_miscellaneous_864957067
- Format
- –
- Schlagworte
- Adenoviruses, Human - metabolism, Amino Acid Sequence, Animals, Antiviral activity, Antiviral Agents - metabolism, Antiviral Agents - pharmacology, antiviral properties, Base Sequence, Bovine interferon lambda, Cattle, cattle diseases, Cell culture, Cell Line, Cloning, Molecular, Expression vectors, FMDV, Foot-and-mouth disease, Foot-and-mouth disease virus, Foot-and-Mouth Disease Virus - drug effects, Gene expression, Gene Expression Regulation, Genetic Therapy, host-pathogen relationships, Human adenovirus, Humans, IFN-λ, IL28B, immune response, Infection, Infectious Disease, Inoculation, Interferon, Interferon stimulated genes, interferons, Interferons - genetics, Interferons - metabolism, Interferons - pharmacology, Livestock, Male, Molecular Sequence Data, Vaccines, Vesicular stomatitis virus