Oncogene, 2010-11, Vol.29 (47), p.6203-6215
2010
Details
- Autor(en) / Beteiligte
- Titel
- Mast cells have a protumorigenic role in human thyroid cancer
- Ist Teil von
- Oncogene, 2010-11, Vol.29 (47), p.6203-6215
- Ort / Verlag
- Basingstoke: Nature Publishing Group
- Erscheinungsjahr
- 2010
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In different human carcinoma types, mast cell infiltrate increases with respect to normal tissue and mast cell density correlates with a bad prognosis. To assess the role of mast cells in human thyroid cancer, we compared the density of tryptase-positive mast cells in 96 papillary thyroid carcinomas (PTCs) versus normal thyroid tissue from 14 healthy individuals. Mast cell density was higher in 95% of PTCs (n=91) than in control tissue. Mast cell infiltrate correlated with extrathyroidal extension (P=0.0005) of PTCs. We show that thyroid cancer cell-line-derived soluble factors induce mast cell activation and chemoattraction in vitro. Different mast cell lines (HMC-1 and LAD2) and primary human lung mast cells induced thyroid cancer cell invasive ability, survival and DNA synthesis in vitro. The latter effect was mainly mediated by three mast-cell-derived mediators: histamine, and chemokines CXCL1/GROα and CXCL10/IP10. We show that xenografts of thyroid carcinoma cells (8505-C) could recruit mast cells injected into the tail vein of mice. Co-injection of human mast cells accelerated the growth of thyroid cancer cell (8505-C) xenografts in athymic mice. This effect was mediated by increased tumor vascularization and proliferation, and was reverted by treating mice with sodium cromoglycate (Cromolyn), a specific mast cell inhibitor. In conclusion, our study data suggest that mast cells are recruited into thyroid carcinomas and promote proliferation, survival and invasive ability of cancer cells, thereby contributing to thyroid carcinoma growth and invasiveness.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-9232eISSN: 1476-5594DOI: 10.1038/onc.2010.348Titel-ID: cdi_proquest_miscellaneous_864953903
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Cell activation, Cell Count, Cell density, Cell Line, Tumor, Cell physiology, Cell Proliferation, Cell Survival, Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes, Cell Transformation, Neoplastic, Cellular biology, Chemokines, CXCL10 protein, Cytokines, DNA biosynthesis, Endocrinopathies, Fundamental and applied biological sciences. Psychology, Genetic aspects, Histamine, Humans, Invasiveness, Male, Malignant tumors, Mast cells, Mast Cells - immunology, Mast Cells - physiology, Medical prognosis, Medical sciences, Mice, Mice, Inbred BALB C, Molecular and cellular biology, Neoplasm Invasiveness, Neoplasm Transplantation, Non tumoral diseases. Target tissue resistance. Benign neoplasms, Papillary thyroid carcinoma, Pathology, Physiological aspects, Prognosis, Risk factors, Sodium cromoglycate, Thyroid cancer, Thyroid diseases, Thyroid Neoplasms - blood supply, Thyroid Neoplasms - metabolism, Thyroid Neoplasms - pathology, Thyroid. Thyroid axis (diseases), Tryptase, Tumors, Vascular Endothelial Growth Factor A - metabolism, Vascularization, Xenografts