Details

Autor(en) / Beteiligte

• Miyamura, Norio
• Nakamura, Takashi
• Goto-Inoue, Naoko
• Zaima, Nobuhiro
• Hayasaka, Takahiro
• Yamasaki, Tokiwa
• Terai, Shuji
• Sakaida, Isao
• Setou, Mitsutoshi
• Nishina, Hiroshi

Titel

Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver

Ist Teil von

• Biochemical and biophysical research communications, 2011-04, Vol.408 (1), p.120-125

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2011

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• ► We report the first use of IMS to analyze the partially hepatectomized liver. ► IMS revealed characteristic changes in species of triglycerides and phospholipids. ► Several species of phospholipids demonstrated distribution gradients in lobules. ► Partial hepatectomy induced changes to expression of genes in adipose tissues. After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.