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A novel non-enzyme glucose amperometric biosensor was fabricated based on biospecific binding affinity of concanavalin A (Con A) for
d-glucose on thionine (TH) modified electrode. TH can be covalently immobilized on potentiostatically activated glassy carbon electrode through Schiff-base reaction. Subsequently, the surface-adherent polydopamine film formed by self-polymerization of dopamine attached to TH and afforded binding sites for the subsequent immobilization of Con A molecules via Michael addition and/or Schiff-base reaction with high stability. Thus, a sensing platform for specific detection towards
d-glucose was established. The binding of Con A towards
d-glucose can be monitored through the decrease of the electrode response of the TH moiety. Due to the high affinity of Con A for
d-glucose and high stability of the resulting sensing platform, the fabricated biosensor exhibited high selectivity, good sensitivity, and wide linear range from 1.0
×
10
−6 to 1.0
×
10
−4
M with a low detection limit of 7.5
×
10
−7
M towards
d-glucose.