Details

Autor(en) / Beteiligte

• Liu, Xin-Guang
• Li, Jin-Lin
• Qin, Ping
• Ren, Juan
• Ma, Shi-Hui
• Sun, Lin
• Shi, Yan
• Ji, Xue-Bin
• Zhu, Yuan-Yuan
• Ma, Dao-Xin
• Guo, Cheng-Shan
• Du, Xin
• Hou, Ming
• Peng, Jun

Titel

Determination of platelet-bound glycoprotein-specific autoantibodies by flow cytometric immunobead assay in primary immune thrombocytopenia

Ist Teil von

• European journal of haematology, 2011-04, Vol.86 (4), p.339-346

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Objectives:  Primary immune thrombocytopenia (ITP) is an autoimmune disorder characterized by premature platelet destruction induced by autoantibodies directed against platelet glycoproteins (GPs). Despite being a clinically important disorder, ITP lacks a feasible diagnostic assay for routine clinical use. This study was meant to evaluate a newly developed flow cytometric immunobead assay for determination of platelet‐bound GP‐specific autoantibodies in comparison with indirect monoclonal antibody‐specific immobilization of platelet antigen (MAIPA) in the diagnosis of ITP. Methods:  Platelet‐bound and plasma GPIIb/IIIa and GPIb/IX autoantibodies were determined by flow cytometric immunobead assay and indirect modified MAIPA, respectively. The average fluorescence level for platelet‐bound, GP‐specific autoantibodies was given as a ratio to three normal controls tested simultaneously. Results:  The median value of platelet‐bound GPIIb/IIIa and GPIb/IX autoantibodies in ITP group were 3.09 (range 0.78, 30.2) and 3.09 (range 0.72, 19.2), respectively, which were significantly higher than non‐ITP group [1.01 (0.67, 5.59) and 1.01 (0.79, 5.56), respectively, P < 0.001] and normal controls [1.02 (0.72, 1.76) and 1.03 (0.79, 1.73), respectively, P < 0.001]. The receiver‐operating characteristics curve analysis showed an area under the curve of 0.895 for GPIIb/IIIa autoantibody and 0.859 for GPIb/IX autoantibody, respectively. Combined detection of GPIIb/IIIa or GPIb/IX autoantibodies by flow cytometric immunobead assay showed a sensitivity of 82.11% for ITP diagnosis. Conclusion:  This study demonstrated that determination of platelet‐bound, GP‐specific autoantibodies by flow cytometric immunobead assay was a convenient, sensitive, and specific test for the differential diagnosis of thrombocytopenic patients.