Lung cancer (Amsterdam, Netherlands), 2011-05, Vol.72 (2), p.229-237
2011
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Minichromosome maintenance (MCM) protein 4 as a marker for proliferation and its clinical and clinicopathological significance in non-small cell lung cancer
- Ist Teil von
- Lung cancer (Amsterdam, Netherlands), 2011-05, Vol.72 (2), p.229-237
- Ort / Verlag
- Oxford: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Background Minichromosome maintenance (MCM) proteins 2–7 form a complex essential for the initiation of DNA replication. In the process to screen expression changes related to growth suppression of non-small cell lung cancer (NSCLC) cells by a cJun dominant-negative mutant, we found that reduced expression of MCM4 was correlated with this growth suppression. Method We determined the relevance of MCM4 in proliferation of NSCLC by downregulating its expression with small-interfering RNA in three NSCLC cell lines. We then immunohistochemically analyzed MCM4 expression in 156 surgically resected NSCLCs to correlate clinicopathologic characteristics. Results MCM4 downregulation reduced proliferation in two cell lines. MCM4 expression was higher in cancer cells than in adjacent normal bronchial epithelial cells ( p < 0.001). High MCM4 expression was correlated with male gender, heavy smoking, poorer differentiation and non-adenocarcinoma histology ( p < 0.001, respectively). High MCM4 expression was also correlated with proliferation markers, Ki-67 and cyclin E expression ( p < 0.001, respectively). MCM4 expression was not associated with survival. Conclusion MCM4 may play an essential role in the proliferation of some NSCLC cells. Taken together with higher expression in NSCLCs and its correlation with clinicopathologic characteristics such as non-adenocarcinoma histology, MCM4 may have potential as a therapeutic target in certain population with NSCLCs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0169-5002eISSN: 1872-8332DOI: 10.1016/j.lungcan.2010.08.020Titel-ID: cdi_proquest_miscellaneous_861787308
- Format
- –
- Schlagworte
- Aged, AP-1, Biological and medical sciences, Biomarkers, Tumor - genetics, Biomarkers, Tumor - metabolism, Carcinoma, Non-Small-Cell Lung - genetics, Carcinoma, Non-Small-Cell Lung - metabolism, Carcinoma, Non-Small-Cell Lung - mortality, Carcinoma, Non-Small-Cell Lung - pathology, Carcinoma, Non-Small-Cell Lung - physiopathology, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cell Growth Processes - genetics, Cell Line, Tumor, Cyclin E - genetics, Cyclin E - metabolism, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Female, Hematology, Oncology and Palliative Medicine, Humans, Immunohistochemistry, JNK Mitogen-Activated Protein Kinases - genetics, Ki-67 Antigen - genetics, Ki-67 Antigen - metabolism, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Lung Neoplasms - mortality, Lung Neoplasms - pathology, Lung Neoplasms - physiopathology, Male, Medical sciences, Minichromosome Maintenance Complex Component 4, Minichromosome maintenance protein 4, Mutation - genetics, Non-small cell lung cancer, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Pneumology, Proliferation, Pulmonary/Respiratory, Respiratory Mucosa - metabolism, Respiratory Mucosa - pathology, RNA, Small Interfering - genetics, Sex Factors, Survival Analysis, TAM67, Tumors, Tumors of the respiratory system and mediastinum