Biochimie, 2011-05, Vol.93 (5), p.899-908
2011
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- Autor(en) / Beteiligte
- Titel
- Attenuation of LPS-induced apoptosis in NGF-differentiated PC12 cells via NF-κB pathway and regulation of cellular redox status by an oxazine derivative
- Ist Teil von
- Biochimie, 2011-05, Vol.93 (5), p.899-908
- Ort / Verlag
- France: Elsevier B.V
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Neuronal cell death due to apoptosis is a common characteristic of neurodegenerative diseases. In this study, we report protective effect of 2-ethoxy-4,5-diphenyl-1,3-oxazine-6-one (EDPOO) against lipopolysaccharide (LPS)-induced cell death in rat pheochromocytoma (PC12) cells, as assessed by MTT test, acridine orange/ethidium bromide staining, determination of Bax, Bcl-2 and caspase-3 levels. We further show that this compound could increase heat shock proteins Hsp-70 and Hsp-32 levels. EDPOO also modulates nuclear levels of Nrf2 and NF-κB, transcription factors that are activated by intracellular reactive oxygen species and/or mediators generated due to chemical exposure of cells. Pretreatment of the cells with this oxazine derivative also increases γ-GCS level, as well as antioxidant enzyme activities, in a dose-dependent manner. Protective effect of this compound could represent a promising approach for treatment of neurodegenerative diseases. ► Pretreatment with EDPOO protected differentiated PC12 cells against LPS-induced apoptosis. ► EDPOO fortified compensatory responses by mounting Nrf2 nuclear level and antioxidant enzymes activity. ► EDPOO attenuated NF-κB activation via suppression of ROS production.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0300-9084eISSN: 1638-6183DOI: 10.1016/j.biochi.2011.01.012Titel-ID: cdi_proquest_miscellaneous_861590680
- Format
- –
- Schlagworte
- Animals, Antioxidants - pharmacology, Apoptosis, Apoptosis - drug effects, bcl-2-Associated X Protein - metabolism, bcl-Associated Death Protein - metabolism, Caspase 3 - metabolism, Catalase - metabolism, Cell Differentiation - drug effects, Cell Survival - drug effects, Cyclooxygenase 2 - metabolism, Glutamate-Cysteine Ligase - metabolism, Heat-Shock Proteins - metabolism, Lipid Peroxidation - drug effects, Lipopolysaccharides - pharmacology, LPS, Nerve Growth Factor - pharmacology, Neurons - cytology, Neurons - drug effects, Neurons - metabolism, NF-E2-Related Factor 2 - metabolism, NF-kappa B - metabolism, NF-κB, Nitric Oxide - metabolism, Oxazine derivative, Oxazines - pharmacology, Oxidation-Reduction, PC12 Cells, Protein Transport - drug effects, Rats, Reactive Oxygen Species - metabolism, Signal Transduction - drug effects, Superoxide Dismutase - metabolism