Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.436-442
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Determination of Ganciclovir and its prodrug Valganciclovir by hydrophilic interaction liquid chromatography–tandem mass spectrometry
- Ist Teil von
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.436-442
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- This manuscript describes the determination of Ganciclovir (GCV), active component of the antiviral drug Valcyte ®, and its ester prodrug Valganciclovir (VGC) in human and rat plasma, using liquid chromatography coupled to tandem mass spectrometry. Protein precipitation with acetonitrile was followed by hydrophilic interaction liquid chromatography on a silica column with 4 min run time. After electrospray ionization, the compounds were detected in positive ion selected reaction monitoring (SRM) mode. The lower limits of quantification (LLOQ) were 16 ng/mL for GCV and 4 ng/mL for VGC in human and rat plasma. Inter-day and intra-day precisions and inaccuracies were below 15% and between 85 and 115%, respectively. Five-fold deuterated GCV and VGC were used as internal standards and compensated for any matrix effect. The method was successfully applied to samples from a rat pharmacokinetic study. The feasibility of blood analysis as dried blood spots (DBS) was investigated.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1570-0232eISSN: 1873-376XDOI: 10.1016/j.jchromb.2010.12.033Titel-ID: cdi_proquest_miscellaneous_861570167
- Format
- –
- Schlagworte
- acetonitrile, Analysis, Analytical, structural and metabolic biochemistry, Animals, antiviral agents, Biological and medical sciences, Blood, blood plasma, Blood Specimen Collection, Chromatography, Chromatography, Liquid - methods, Dried blood spots (DBS), Drug Stability, Fundamental and applied biological sciences. Psychology, Ganciclovir (GCV), Ganciclovir - analogs & derivatives, Ganciclovir - blood, General pharmacology, hematologic tests, Human, Humans, Hydrophilic interaction liquid chromatography (HILIC), Hydrophobic and Hydrophilic Interactions, Ionization, Liquid chromatography, Liquid chromatography–tandem mass spectrometry (LC–MS/MS), Mass spectrometry, Medical sciences, monitoring, pharmacokinetics, Pharmacology. Drug treatments, Plasma, Rats, Rats, Sprague-Dawley, Rats, Wistar, Reproducibility of Results, Run time (computers), Sensitivity and Specificity, silica, tandem mass spectrometry, Tandem Mass Spectrometry - methods, Valcyte, Valganciclovir (VGC)