Details

Autor(en) / Beteiligte

• Bhope, S. G.
• Kuber, V. V.
• Nagore, D. H.

Titel

Validated HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula Linn

Ist Teil von

• Acta chromatographica, 2010-09, Vol.22 (3), p.481-489

Erscheinungsjahr

2010

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• This paper describes a simple, precise, and accurate HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula whole plant extract. Chromatographic separation of the sample extract was performed on aluminium foil plates coated with silica gel 60 F sub(254) as stationary phase. The mobile phase was toluene-ethyl acetate-methanol-formic acid 8:10:5:2 (v/v). Densitometric evaluation of the separated bands was performed at 270 nm. Sennosides A and B and kaempferol were satisfactorily resolved at R sub(F) 0.22 plus or minus 0.05, 0.19 plus or minus 0.05, and 0.81 plus or minus 0.05, respectively. Recovery of sennosides A and B and kaempferol from Cassia fistula extract was 98.03, 98.74, and 99.08%, respectively. The method was validated for specificity, accuracy, linearity (100-400 ng per band), and precision (instrument precision in the range 1.03-1.33 and method precision in the range of 1.31-1.75) in accordance with ICH guidelines.