Biometals, 2011-04, Vol.24 (2), p.181-191
2011
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- Autor(en) / Beteiligte
- Titel
- Heme oxygenase is involved in cobalt chloride-induced lateral root development in tomato
- Ist Teil von
- Biometals, 2011-04, Vol.24 (2), p.181-191
- Ort / Verlag
- Dordrecht: Dordrecht : Springer Netherlands
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In animals, heme oxygenase (HO), a rate-limiting enzyme responsible for carbon monoxide (CO) production, was regarded as a protective system maintaining cellular homeostasis. It was also established that metal ions are powerful HO-inducing agents and cobalt chloride (CoCl₂) was the first metal ion identified with an inducing property. Previous study suggests that CoCl₂ stimulates adventitious root formation in tomato and cucumber cuttings. In this test, we discover that both CoCl₂ and an inducer of HO-1, hemin, could lead to the promotion of lateral root development, as well as the induction of HO-1 protein expression, HO activity, or LeHO-1/2 transcripts, in lateral root initiation zone of tomato seedlings. The effect is specific for HO since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) blocked the above actions of CoCl₂, and the inhibitory effect was reversed partially when 50% CO aqueous solution was added. However, the addition of ascorbic acid (AsA), a well-known antioxidant, exhibited no obvious effect on lateral root formation. Molecular evidence further showed that CoCl₂-induced the up-regulation of target genes responsible for lateral root formation, including LeCDKA1, LeCYCA2;1, and LeCYCA3;1, was suppressed differentially by ZnPPIX. And these decreases were reversed further by the addition of CO. All together, these results suggest a novel role for HO in the CoCl₂-induced tomato lateral root formation.
- Sprache
- Englisch; Niederländisch
- Identifikatoren
- ISSN: 0966-0844eISSN: 1572-8773DOI: 10.1007/s10534-010-9386-1Titel-ID: cdi_proquest_miscellaneous_857288643
- Format
- –
- Schlagworte
- Ascorbic Acid - pharmacology, Biochemistry, Biomedical and Life Sciences, Blotting, Western, Carbon monoxide, Cell Biology, Cell cycle, Cell cycle regulatory gene, Cellular, Chlorides, Cobalt, Cobalt - pharmacology, Cobalt chloride, Enzymes, Heme oxygenase, Heme Oxygenase (Decyclizing) - genetics, Heme Oxygenase (Decyclizing) - metabolism, Inhibitors, Ions, Lateral root development, Life Sciences, Lycopersicon esculentum - drug effects, Lycopersicon esculentum - enzymology, Lycopersicon esculentum - metabolism, Medicine/Public Health, Metal ions, Metals, Microbiology, Pharmacology/Toxicology, Plant biology, Plant Physiology, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Roots - drug effects, Plant Roots - enzymology, Plant Roots - metabolism, Reverse Transcriptase Polymerase Chain Reaction, Roots, Seedlings - drug effects, Seedlings - enzymology, Seedlings - metabolism, Solanum lycopersicum var. lycopersicum, Tomatoes, Zinc