Details

Autor(en) / Beteiligte

• McDermott, Geoffrey P.
• Noonan, Laura K.
• Mnatsakanyan, Mariam
• Shalliker, R. Andrew
• Conlan, Xavier A.
• Barnett, Neil W.
• Francis, Paul S.

Titel

High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay: Methodological considerations and application to complex samples

Ist Teil von

• Analytica chimica acta, 2010-08, Vol.675 (1), p.76-82

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2010

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• An improved post-column 2,2′-diphenyl-1-picrylhydrazyl (DPPH ) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH response were studied in a univariate approach. Optimum conditions were found to be: 5 × 10 −5 M DPPH reagent prepared in a 75% methanol: 25% 40 mM citric acid–sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2 m × 0.25 mm i.d. PEEK tubing; detection at 521 nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies.