Details

Autor(en) / Beteiligte

• Mohamad, Siti Norita
• Ramanan, Ramakrishnan Nagasundara
• Mohamad, Rosfarizan
• Ariff, Arbakariya B.

Titel

Improved mannan-degrading enzymes’ production by Aspergillus niger through medium optimization

Ist Teil von

• New biotechnology, 2011-02, Vol.28 (2), p.146-152

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL −1) was much higher than those observed on other carbon substrates, locust bean gum (1148 nkat mL −1), α-cellulose (10.7 nkat mL −1), glucose (8.8 nkat mL −1) and carboxymethylcellulose (4.6 nkat mL −1). For fermentation using GG as a carbon source, bacteriological peptone gave the highest β-mannanase activity (1744 nkat mL −1) followed by peptone from meat (1168 nkat mL −1), yeast extract (817 nkat mL −1), ammonium sulphate (241 nkat mL −1), ammonium nitrate (113 nkat mL −1) and ammonium chloride (99 nkat mL −1) when used as a nitrogen source. The composition of bacteriological peptone and initial pH of the medium were further optimized using response surface methodology (RSM). Medium consisted of 21.3 g L −1 GG and 57 g L −1 peptone with initial culture pH of 5.5 was optimum for β-mannanase production (2063 nkat mL −1) by A. niger. The β-mannanase production obtained in this study using A. niger was significantly higher than those reported in the literature.