Molecular genetics and genomics : MGG, 2009-11, Vol.282 (5), p.503-516
2009
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- Autor(en) / Beteiligte
- Titel
- Functional characterization of the Arabidopsis bHLH92 transcription factor in abiotic stress
- Ist Teil von
- Molecular genetics and genomics : MGG, 2009-11, Vol.282 (5), p.503-516
- Ort / Verlag
- Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In our previous microarray analysis of NaCl-treated Arabidopsis roots, we identified a basic-helix-loop-helix (bHLH) transcription factor, bHLH92 (At5g43650), as one of the transcripts showing the greatest fold-increase in abundance upon NaCl exposure. Here, we characterize the role of bHLH92 in the context of abiotic stress physiology and hormone responses. We observed that bHLH92 transcript abundance increases in response to NaCl, dehydration, mannitol, and cold treatments, and compared these responses to those of two closely related genes: bHLH41 and bHLH42. The NaCl-inducibility of bHLH92 was only partially dependent on abscisic acid (ABA) biosynthesis and SALT OVERLY SENSITIVE2 (SOS2) pathways. As compared to WT, root elongation of bhlh92 mutants was more sensitive to mannitol, and these mutants also showed increased electrolyte leakage following NaCl treatments. Overexpression of bHLH92 moderately increased the tolerance to NaCl and osmotic stresses. Finally, we identified at least 19 putative downstream target genes of bHLH92 under NaCl treatment using an oligonucleotide microarray. Together these data show that bHLH92 functions in plant responses to osmotic stresses, although the net contribution of bHLH92-regulated genes to stress tolerance appears relatively limited in proportion to what might be expected from its transcript expression pattern.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1617-4615eISSN: 1617-4623DOI: 10.1007/s00438-009-0481-3Titel-ID: cdi_proquest_miscellaneous_856760618
- Format
- –
- Schlagworte
- Abiotic stress, Abscisic acid, Abscisic Acid - pharmacology, Adaptation, Physiological - drug effects, Adaptation, Physiological - genetics, Amino acids, Animal Genetics and Genomics, Arabidopsis, Arabidopsis - drug effects, Arabidopsis - genetics, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Basic Helix-Loop-Helix Transcription Factors - genetics, Basic Helix-Loop-Helix Transcription Factors - metabolism, Biochemistry, Biomedical and Life Sciences, Biosynthesis, Cell Nucleus - drug effects, Cell Nucleus - metabolism, Cold, DNA, Bacterial - genetics, Electrolytes, Gene Expression Profiling, Gene Expression Regulation, Plant - drug effects, Genes, Genes, Plant, Genomics, Human Genetics, Hydrogen Peroxide - metabolism, Hydroponics, Life Sciences, Microbial Genetics and Genomics, Mutagenesis, Insertional - genetics, Mutation - genetics, Oligonucleotide Array Sequence Analysis, Organ Specificity - drug effects, Original Paper, Osmosis - drug effects, Plant Genetics and Genomics, Protein Transport - drug effects, RNA, Messenger - genetics, RNA, Messenger - metabolism, Salinity, Signal Transduction - drug effects, Signal Transduction - genetics, Sodium Chloride - pharmacology, Stress, Physiological - drug effects, Stress, Physiological - genetics, Transcription factors, Transcription Factors - genetics, Transcription Factors - metabolism