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Disposable screen-printed biosensors have been successfully employed in the development of analytical methods that respond to the growing need to perform rapid “in situ” analyses. Thus, the early detection of microorganisms, which plays an important role in the prevention of human health problems, animals and plants epidemics, has been carried out using this kind of devices. Moreover, microorganisms have been used as biological sensing elements in the development of sensitive microbial biosensors for the analysis of different analytes of interest. This review presents the electrochemical application of disposable screen-printed biosensors in the microbiology field.