The international journal of biochemistry & cell biology, 2011-04, Vol.43 (4), p.632-641
2011
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- Autor(en) / Beteiligte
- Titel
- Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells
- Ist Teil von
- The international journal of biochemistry & cell biology, 2011-04, Vol.43 (4), p.632-641
- Ort / Verlag
- Netherlands: Elsevier Ltd
- Erscheinungsjahr
- 2011
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- Plexin-B1, the receptor for Sema4D, has been reported to trigger multiple and sometimes opposing cellular responses in various types of tumor cells. It has been implicated in the regulation of tumor-cell survival, proliferation, angiogenesis, invasion and metastasis. However, the plexin-B1 gene expression and its regulatory mechanism in cervical cancer remain unclear. The present study shows that plexin-B1 is over-expressed in cervical tumor tissues compared to normal cervical tissues by immunohistochemistry, Western blotting and quantitative RT-PCR. The expression of plexin-B1 is significantly associated with cervical tumor metastasis and invasion according to the analysis of the clinicopathologic data. Plexin-B1 also promotes proliferation, migration and invasion in human cervical cancer HeLa cells. We also found that the plexin-B1 levels are inversely correlated with miR-214 amounts in both cervical cancer tissues and HeLa cells. And miR-214 expression level is also associated with metastasis and invasion of cervical tumor. Furthermore, we demonstrate that plexin-B1 is inhibited by miR-214 through a miR-214 binding site within the 3′UTR of plexin-B1 in HeLa cells. Ectopic expression of miR-214 could inhibit the proliferation capacity, migration and invasion ability of HeLa cells. Our findings suggest that plexin-B1, a target of miR-214, may function as an oncogene in human cervical cancer HeLa cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1357-2725eISSN: 1878-5875DOI: 10.1016/j.biocel.2011.01.002Titel-ID: cdi_proquest_miscellaneous_854378558
- Format
- –
- Schlagworte
- 3' untranslated regions, angiogenesis, Base Sequence, binding sites, Biochemistry, Biology, Cancer, Cell Movement - genetics, Cell Proliferation, Control, Female, Gene expression, Gene Expression Regulation, Neoplastic - genetics, Gene Knockdown Techniques, gene overexpression, Growth, HeLa Cells, Human, Humans, immunohistochemistry, Invasion, metastasis, MicroRNAs - genetics, MicroRNAs - metabolism, Migration, miR-214, neoplasm cells, Neoplasm Invasiveness - genetics, Nerve Tissue Proteins - deficiency, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - metabolism, oncogenes, Plexin-B1, Receptors, Cell Surface - deficiency, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, reverse transcriptase polymerase chain reaction, Survival, tissues, Tumors, Up-Regulation - genetics, uterine cervical neoplasms, Uterine Cervical Neoplasms - genetics, Uterine Cervical Neoplasms - pathology, Western blotting