Details

Autor(en) / Beteiligte

• Figueira, Rita de Cássia S., M.Sc
• Braga, Daniela P.A.F., M.Sc
• Setti, Amanda S., B.Sc
• Iaconelli, Assumpto, M.D
• Borges, Edson, M.D., Ph.D

Titel

Morphological nuclear integrity of sperm cells is associated with preimplantation genetic aneuploidy screening cycle outcomes

Ist Teil von

• Fertility and sterility, 2011-03, Vol.95 (3), p.990-993

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective To examine the effect of sperm morphology on embryo development at the chromosomal level. Design Prospective study. Setting Assisted fertilization center. Patient(s) Couples who underwent IVF-PGS cycle, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 60) or intracytoplasmic morphologically selected sperm injection (IMSI; n = 60). Intervention(s) IVF in conjunction with preimplantation genetic screening (PGS). Main Outcome Measure(s) Sperm nuclear morphology at high-magnification ICSI and incidence of aneuploidy in derived embryo. Result(s) There was a significantly increased incidence for sex chromosome aneuploidy in ICSI embryos when compared with IMSI embryos (23.5% vs. 15.0%, respectively). High-magnification sperm selection was associated with a significantly lower risk of sex chromosome abnormalities (odds ratio [OR], 0.57; confidence interval [CI], 0.37–0.90). The incidence of chaotic embryos was also significantly higher with the ICSI procedure (27.5% vs. 18.8%), while the IMSI procedure was associated with a significantly lower risk of chaotic embryos (OR, 0.64; CI, 0.43–0.96). Moreover, the cycle cancellation rate was significantly higher in ICSI cycles (11.8% vs. 2.5%). High-magnification sperm selection was a significant predictor of the likelihood of cycle cancellation (OR, 0.26; CI, 0.11–0.62). Conclusion(s) Spermatozoa free of nuclear morphological malformations were found to be significantly associated with the lower incidence of aneuploidy in derived embryos, resulting in lower rates of cycle cancellation.