Details

Autor(en) / Beteiligte

• Itoh, N.
• Itagaki, T.
• Kawabata, T.
• Konaka, T.
• Muraoka, N.
• Saeki, H.
• Kanai, K.
• Chikazawa, S.
• Hori, Y.
• Hoshi, F.
• Higuchi, S.

Titel

Prevalence of intestinal parasites and genotyping of Giardia intestinalis in pet shop puppies in east Japan

Ist Teil von

• Veterinary parasitology, 2011-02, Vol.176 (1), p.74-78

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2011

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The current study examined the prevalence of intestinal parasites and genotypes of Giardia intestinalis in puppies from nine pet shops in east Japan. Fresh fecal samples from 1794 puppies (≦3 months old) were collected on one occasion. Giardia spp. was examined for specific coproantigen using ELISA kit (SNAP ® Giardia, IDEXX Laboratories, Inc., USA). Other intestinal parasites were detected microscopically using the formalin-ethyl acetate sedimentation technique. Genotyping was determined for the random 29 stool samples identified as Giardia spp. positive using PCR and direct sequencing of the glutamate dehydrogenase ( gdh) gene. Overall prevalence of protozoan Giardia spp. and Cystoisospora spp. revealed 23.4% and 11.3%, respectively. Prevalence of ascarids, Strongyloides spp. and hookworms were recorded 1.8%, 1.1% and 0.1%, respectively. Protozoan Giardia spp. and Cystoisospora spp., thus, represent important pathogens among pet shop puppies. All genotyped G. intestinalis isolates were belonged to assemblage C or D, identified as dog-specific genotypes. Zoonotic assemblage A and B were not demonstrated. The result suggests that the risk of zoonotic transmission of G. intestinalis from pet shops puppies to humans may be quite low in Japan.