Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.457-460
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system
- Ist Teil von
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.457-460
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1570-0232eISSN: 1873-376XDOI: 10.1016/j.jchromb.2010.12.030Titel-ID: cdi_proquest_miscellaneous_850563238
- Format
- –
- Schlagworte
- Analysis, Analysis of Variance, Analytical, structural and metabolic biochemistry, Aqueous two-phase system, Biological and medical sciences, Canavalia - chemistry, Canavalia ensiformis, Chemical Fractionation - methods, Chromatography, Citrates, Citric Acid - chemistry, concanavalin A, Concanavalin A - chemistry, Concanavalin A - isolation & purification, Downstream processing, Electrophoresis, Polyacrylamide Gel, Extraction, Fundamental and applied biological sciences. Psychology, General pharmacology, Hydrogen-Ion Concentration, Lectin purification, Lectins, Medical sciences, Molecular Weight, Partitions, Pharmacology. Drug treatments, Plant Extracts - chemistry, polyacrylamide gel electrophoresis, polyethylene glycol, Polyethylene Glycols - chemistry, Purification, Seeds, Seeds - chemistry