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Based on the theory of Housner, the transverse seismic response of beam aqueduct considering fluid-structure coupling is established. With the variation of aqueduct cross-section ratio of depth to width, the aqueduct transverse seismic response change. The transverse seismic response of a large-scale aqueduct in several work condition are calculated. It shows that the transverse seismic response is greatly influenced by the water mass in the aqueduct, but the shaking water play a TLD role. ff the whole water is appended aqueduct body, it will magnify seismic inertia action. When aqueduct cross-section is selected, the influence of ratio of depth and width to pier seismic response should be considered in order to reduce seismic action.