Details

Autor(en) / Beteiligte

• Pinchuck, Paul
• Fishman, Marvin
• Adler, Frank L.
• Maurer, Paul H.

Titel

Antibody Formation: Initiation in "Nonresponder" Mice by Macrophage Synthetic Polypeptide RNA

Ist Teil von

• Science (American Association for the Advancement of Science), 1968-04, Vol.160 (3824), p.194-195

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

1968

Quelle

MEDLINE

Beschreibungen/Notizen

• The RNA extracted from normal peritoneal macrophages exposed to a linear, random synthetic polypeptide, Glu$^{60}$Ala$^{30}$Tyr$^{10}$, initiated an immune response in C57B1/6J mice, although this strain responds very poorly to the antigen itself. From 10 to 150 micrograms of RNA obtained from mouse, rat, or rabbit macrophages was injected intraperitoneally into recipient mice, and specific antibody was detectable by passive hemagglutination 3 to 4 weeks later. Treatment of the RNA with ribonuclease destroyed its ability to initiate a specific immune response. The RNA contained by weight 0.02 percent of the (specific) antigen. The RNA obtained from cells incubated with a second polypeptide, Glu$^{36}$Lys$^{24}$Ala$^{40}$, initiated a response specific for this polymer. This RNA even when incubated in vitro with Glu$^{60}$Ala$^{30}$Tyr$^{10}$ failed to initiate antibody formation specific for Glu$^{60}$Ala$^{30}$Tyr$^{10}$.