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Effect of Cycloheximide on the Synthesis and Processing of 5S RNA in HeLa Cells
Ist Teil von
Journal of biochemistry (Tokyo), 1977-02, Vol.81 (2), p.451-459
Ort / Verlag
England: Oxford University Press
Erscheinungsjahr
1977
Quelle
MEDLINE
Beschreibungen/Notizen
The synthesis and incorporation into ribosomes of 5S RNA were investigated under conditions of protein synthesis inhibition by cycloheximide. Within 1 h after addition of cycloheximide to the culture medium, the synthesis of 5S RNA in HeLa cells, as measured by the pulse labeling of total 5S RNA, was inhibited by 25–30 per cent, but the degree of inhibition did not further increase for several hours. The synthesis of 4S RNA was much less markedly inhibited, especially at earlier time periods. Although the appearance of labeled 5S RNA in cytoplasmic ribosomes after chase was inhibited by approximately 60% or more, this presumably reflects the inhibition of 60S ribo-somal subunit formation by cycloheximide. Some utilization of preformed 5S RNA for new ribosomes was suggested after resumption of protein synthesis. In confirmation of the results of Kumar and Wu (l), a low dose of actinomycin D inhibited the appearance of 28S RNA in the cytoplasm rather specifically, whereas cycloheximide showed an apparently opposite differential effect.