Details

Autor(en) / Beteiligte

• Otagiri, M.
• Perrin, J.H.

Titel

Circular dichroic investigations of the binding of salicylate and related compounds to human serum albumin

Ist Teil von

• Biochemical pharmacology, 1977-02, Vol.26 (4), p.283-288

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

1977

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The binding of salicylate and some related compounds has been investigated by circular dichroism. The binding constants, where possible, were determined by direct titration, otherwise by their ability to displace salicylate. The binding constants for the first two sites for salicylate were found to be 1.05 × 10 5 1. mole −1 and 5.10 × 10 3 1. mole −1. The induced circular dichroism of salicylate was diminished by addition of acetate ions. Attempts to correlate binding constants with partition coefficients and Hammett σ values suggested hydrophobic and electrostatic forces to be involved in the binding of these benzoic acid derivatives. Aspirin, indomethacin and phenylbutazone appeared to share the primary site with salicylate.