Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Measurement Of The Staphylococcal Epidermolytic Toxin: A Comparison Of Bioassay, Radial Immunodiffusion, And Radioimmunoassay
Ist Teil von
Journal of investigative dermatology, 1976-10, Vol.67 (4), p.526-531
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
1976
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Three methods of measuring the epidermolytic toxin of Staphylococcus aureus-bioassay in newborn mice, radial immunodiffusion, and radioimmunoassay-were compared for reproducibility, specificity, and sensitivity. The bioassay is highly specific and remains the only functional assay. It is reproducible only if newborn mice of the same age are used. The time required for epidermolysis follows a dose-response relationship only if concentrations of toxin large enough to cause peeling in 90 mm or less are used. This limits the sensitivity of the bioassay to about 5 μg per ml.
Single radial immunodiffusion in agar is a specific and reproducible assay method, but its sensitivity is also about 5 μg per ml.
A radioimmunoassay was established by the Farr technique using purified epidermolysin radiolabeled with 125 iodine. This assay was highly reproducible and specific. The staphylococcal products, α-toxin and enterotoxins A and B, did not cross-react with anti-epidermolysin antibodies. The sensitivity of the radioimmunoassay is 20 ng per ml.