Details

Autor(en) / Beteiligte

• Albrechtsen, H
• Hammer-Jespersen, K
• Munch-Petersen, A
• Fiil, N

Titel

Multiple regulation of nucleoside catabolizing enzymes: effects of a polar dra mutation on the deo enzymes

Ist Teil von

• Molecular & general genetics, 1976-01, Vol.146 (2), p.139-145

Ort / Verlag

Germany

Erscheinungsjahr

1976

Quelle

MEDLINE

Beschreibungen/Notizen

• Strains with an amber, polar mutation in the dra1 gene have been isolated. The mutation was introduced into a set of isogenic strains, wild type or with concurrent regulatory mutations, and further characterized by suppression and heat inactivation experiments. The effect of the polar dra mutation on the three remaining genes of the deo operon, the tpp, drm and pup genes, was determined by estimating the enzyme levels in the various dra-mutants. The effect was found to be non-coordinate, indicating the formation in the cells of two types of transcripts: A tetracistronic unit, containing the message from all four genes, and a dicistronic unit, covering the two distal genes only.