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Primary culture and maintenance of steroid-secreting human placenatal monolayers
Ist Teil von
In vitro, 1976-02, Vol.12 (2), p.115-119
Ort / Verlag
United States
Erscheinungsjahr
1976
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
A simple method is described for primary culture and for maintenance of hormone-producing cells from normal human placenta. A consistent yield of cells was obtained and an average survival of 3 to 4 months in culture using 1 mm3 explants from the most vascular area of the placentas. These explants were placed in a variety of culture media in 30 ml flasks and incubated at 37 degrees C in an atmosphere of 5% CO2 and 95% air. The best yields in terms of cell growth were observed with Eagle's MEM (minimum essential medium) with supplements of horse serum and fetal calf serum or human cord serum. (Ham's F-10 with supplement of horse serum and fetal calf serum supports growth for the longest period and media containing human cord serum had the best yield of steroids.