Details

Autor(en) / Beteiligte

• Katsuki, H.
• Ariga, N.
• Katsuki, F.
• Nagai, J.
• Egashira, S.
• Tanaka, S.

Titel

Enzymic hydration of mesaconate by Pseudomonas fluorescens

Ist Teil von

• Biochimica et biophysica acta, 1962-01, Vol.56, p.545-551

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

1962

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Sonicated extract of Pseudomonas fluorescens could, in the presence of Fe 2+, reversibly hydrate mesaconate to produce d-citamalate. This enzyme was partially purified by protamine treatment, heating and ammonium sulfate fractionation. In the purified preparation, the reaction proceeded stoichiometrically and reached the equilibrium state in which the proportion of d-citramalate to mesaconate was about 6:1. K m of this enzyme for d-citramalate was 5·10 −4 M and its optimum pH was 8.2. It was inhibited by various metal ions, iodoacetate and cysteine.