Details

Autor(en) / Beteiligte

• Kowit, J D
• Fulton, C

Titel

Purification and Properties of Flagellar Outer Doublet Tubulin from Naegleria gruberi and a Radioimmune Assay for Tubulin

Ist Teil von

• The Journal of biological chemistry, 1974-06, Vol.249 (11), p.3638-3646

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

1974

Quelle

MEDLINE

Beschreibungen/Notizen

• Outer doublet tubulin has been purified from flagella of differentiated cells of the amebo-flagellate Naegleria gruberi . The flagellar tubulin is similar to other tubulins in molecular weight (55,000), amino acid composition, electrophoretic mobility, and nucleotide composition. However, the protein contains 2 moles of guanine nucleotide per 55,000 g of protein, twice that usually found in tubulin. A rabbit antiserum against flagellar tubulin was shown to contain antibodies specific for tubulin. Purified tubulin iodinated with 125 I was used in a radioimmune assay to measure levels of flagellar tubulin antigen in cells. The measurements indicate that the antigen increases 35- to 55-fold during differentiation of amebae to flagellates.