Details

Autor(en) / Beteiligte

• Piret, Gaëlle
• Desmet, Rémi
• Diesis, Eric
• Drobecq, Hervé
• Segers, Jérome
• Rouanet, Carine
• Debrie, Anne-Sophie
• Boukherroub, Rabah
• Locht, Camille
• Melnyk, Oleg

Titel

Chips from Chips: Application to the Study of Antibody Responses to Methylated Proteins

Ist Teil von

• Journal of proteome research, 2010-12, Vol.9 (12), p.6467-6478

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Peptide microarrays are useful tools for the characterization of humoral responses against peptide antigens. The study of post-translational modifications requires the printing of appropriately modified peptides, whose synthesis can be time-consuming and expensive. We describe here a method named “chips from chips”, which allows probing the presence of antibodies directed toward modified peptide antigens starting from unmodified peptide microarrays. The chip from chip concept is based on the modification of peptide microspots by simple chemical reactions. The starting peptide chip (parent chip) is covered by the reagent solution, thereby allowing the modification of specific residues to occur, resulting in the production of a modified peptide chip (daughter chip). Both parent and daughter chips can then be used for interaction studies. The method is illustrated using reductive methylation for converting lysines into dimethyllysines. The rate of methylation was studied using specific antibodies and fluorescence detection, or surface-assisted laser desorption ionization mass spectrometry. This later technique showed unambiguously the efficient methylation of the peptide probes. The method was then used to study the humoral response against the Mycobacterium tuberculosis heparin-binding hemagglutinin, a methylated surface-associated virulence factor and powerful diagnostic and protective antigen.