Details

Autor(en) / Beteiligte

• Stahl, Franklin W.
• McMilin, Kenneth D.
• Stahl, Mary M.
• Nozu, Yuzo

Titel

An Enhancing Role for DNA Synthesis in Formation of Bacteriophage Lambda Recombinants

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1972-12, Vol.69 (12), p.3598-3601

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1972

Quelle

MEDLINE

Beschreibungen/Notizen

• Recombination in some intervals of the map of phage lambda is associated with more DNA synthesis than in other intervals. Blockage of DNA synthesis by high temperature in a host temperature-sensitive for DNA synthesis results in the relative reduction of recombinant frequencies in those regions having the larger amounts of recombination-associated synthesis. Reduction of DNA synthesis at normal temperatures by a combination of the bacterial mutation and a mutation in one of the phage genes required for DNA synthesis has the same consequence. Therefore, DNA synthesis enhances recombinant particle formation more in some map intervals than in others.