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Details

Autor(en) / Beteiligte
Titel
Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry
Ist Teil von
  • Proteomics. Clinical applications, 2007-07, Vol.1 (7), p.650-660
Ort / Verlag
Weinheim: WILEY-VCH Verlag
Erscheinungsjahr
2007
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
  • We have established and validated a protocol for the peptidomic analysis of rat urine using CE coupled to MS (CE‐MS). In the first experiments, the reproducibility of the CE‐MS set‐up and of the established preparation procedure were assessed. To establish a first rat urinary peptidome map, samples were also analyzed using CE‐FT‐ICR. The subsequent analysis of independent samples from two different strains (WISTAR and CD) indicated strain‐specific differences, which were validated in a blinded assessment. MS/MS revealed the presence of specific fragments from well‐known urinary rat peptides, such as collagens, alpha‐1‐antitrypsin, and serum albumin. The CE‐MS‐based peptidomics platform may provide novel insights into body fluids of animal models, such as rat or mice. Together with peptide identification, the technology appears to be an excellent, complimentary, and non‐invasive tool to analyze toxicological or other (patho)physiological effects of pharmaceutical compounds in animal models.
Sprache
Englisch
Identifikatoren
ISSN: 1862-8346
eISSN: 1862-8354
DOI: 10.1002/prca.200700195
Titel-ID: cdi_proquest_miscellaneous_816529903
Format
Schlagworte
Biomarker, CE-MS, Rat, Urine

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