Journal of microbiological methods, 2010-11, Vol.83 (2), p.254-256
2010
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Details
- Autor(en) / Beteiligte
- Titel
- A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential
- Ist Teil von
- Journal of microbiological methods, 2010-11, Vol.83 (2), p.254-256
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We developed a homogenous microtiter based assay using the cationic dye 3, 3′-Diethyloxacarbocyanine iodide, DiOC 2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti- Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0167-7012eISSN: 1872-8359DOI: 10.1016/j.mimet.2010.08.012Titel-ID: cdi_proquest_miscellaneous_815542459
- Format
- –
- Schlagworte
- Anti-Bacterial Agents - pharmacology, Assay, Bacteriological methods and techniques used in bacteriology, Bacteriological Techniques - methods, Bacteriology, Biological and medical sciences, Carbocyanines - metabolism, Escherichia coli - drug effects, Fundamental and applied biological sciences. Psychology, High-Throughput Screening Assays - methods, Inhibitory Concentration 50, Membrane potential, Membrane Potentials - drug effects, Microbiology, Microtiter, Staphylococcus aureus, Staphylococcus aureus - drug effects