Applied biochemistry and biotechnology, 2000, Vol.84 (1-9), p.267-276
2000
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- Autor(en) / Beteiligte
- Titel
- Endocellulase and exocellulase activities two Streptomyces strains isolated from a forest soil
- Ist Teil von
- Applied biochemistry and biotechnology, 2000, Vol.84 (1-9), p.267-276
- Ort / Verlag
- Heidelberg: Springer
- Erscheinungsjahr
- 2000
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Two Streptomyces strains, M7a and M23, from a Brazilian forest soil were evaluated for the cellulase production of their supernatants after growth in a microcrystalline cellulose medium, using carboxymethylcellulose and filter paper as substrates at different temperatures and pH values. Endoglucanase and exoglucanase activities were compared to a commercial Trichoderma reesei cellulase using fluorogenic conjugated substrates. Similar specific activities were observed for the enzyme preparations of strain M23 and T. reesei. For M7a the activities were about seven times higher than those obtained for T. reesei. Extracellular or cell-associated cellobiase activities were not detected in both strains.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0273-2289eISSN: 1559-0291, 0273-2289DOI: 10.1385/ABAB:84-86:1-9:267Titel-ID: cdi_proquest_miscellaneous_815536142
- Format
- –
- Schlagworte
- beta-glucosidase, Biological and medical sciences, Biology of microorganisms of confirmed or potential industrial interest, Biotechnology, Brazil, carboxymethylcellulose, Carboxymethylcellulose Sodium, Cellulase - metabolism, Cellulose, endo-1,4-beta-glucanase, Fermentation, Forest soils, Fundamental and applied biological sciences. Psychology, Hypocrea jecorina, Kinetics, Miscellaneous, Mission oriented research, Soil Microbiology, Streptomyces, Streptomyces - enzymology, Streptomyces - isolation & purification, Substrate Specificity, temperature, Trichoderma - enzymology, Trichoderma reesei