Details

Autor(en) / Beteiligte

• Roseto, Alberto
• Pothier, Pierre
• Guillemin, Marie-Claude
• Peries, Jorge
• Breitburd, Francoise
• Bonneaud, Nathalie
• Orth, Gerard

Titel

Monoclonal Antibodies to the Major Capsid Protein of Human Papillomavirus Type 1

Ist Teil von

• Journal of general virology, 1984-08, Vol.65 (8), p.1319-1324

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

1984

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Unité INSERM 107, LOI CNRS, Institut de Recherches sur les Maladies du Sang, Höpital Saint-Louis, 75475 Paris Cédex 10 2 Laboratoire de Bactériologie-Virologie, Faculté de Médecine, 21000 Dijon and 3 Unité INSERM 190, Institut Pasteur, 75724 Paris Cédex 15, France Two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (HPV-1) were isolated. The monoclonal antibodies detected HPV-1 antigens in frozen sections of HPV-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with HPV-1 particles in an immunodiffusion test. The two monoclonal antibodies recognized the major structural viral polypeptide, with a molecular weight of 54000, and a minor polypeptide, with a molecular weight of 76000, in both the dissociated viral particles and in the wart extracts. Keywords: HPV-1, capsid protein, monoclonal antibodies Received 6 February 1984; accepted 5 April 1984.