Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Diagnostic value of plasmid analyses and assays for virulence in Yersinia enterocolitica
Ist Teil von
Diagnostic microbiology and infectious disease, 1984-01, Vol.2 (3), p.219-228
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
1984
Link zum Volltext
Quelle
EZB-NALI5-00465 Elsevier Archive NL
Beschreibungen/Notizen
The possession of a 42- to 48-megadalton plasmid alone does not appear to be predictive of virulence in
Yersinia
species. Twelve of 100
Yersinia enterocolitica
strains contained a 42 to 48-megadalton plasmid, and 4 of 30
Y. enterocolitica
-like strains contained a 42- to 48-megadalton plasmid. Seven strains of
Y. enterocolitica
contained the 42- to 48-megadalton plasmid plus an 82-megadalton plasmid, and these were the only study strains lethal for mice. Based on restriction endonuclease digestion, the 42- to 48-megadalton plasmid DNA from these seven strains were similar and were not similar to the 42- to 48-megadalton plasmids present in the other nine strains. The ability to invade guinea pig eye tissues, calcium dependency, autoagglutination, and colonial morphology at 37°C were also associated with plasmid DNA, but the relationships were either variable or not reciprocal. Neither tissue culture invasiveness nor heat-stable toxin production was associated with plasmid DNA. It was concluded that biochemical speciation and a total plasmid profile in combination with enzyme digests are predictive of virulence in
Y. enterocolitica
as it is measured by mouse lethality.