Details

Autor(en) / Beteiligte

• Donson, Jonathan
• Accotto, Gian Paolo
• Boulton, Margaret I.
• Mullineaux, Philip M.
• Davies, Jeffrey W.

Titel

The nucleotide sequence of a geminivirus from Digitaria sanguinalis

Ist Teil von

• Virology (New York, N.Y.), 1987-11, Vol.161 (1), p.160-169

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

1987

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The encapsidated single-stranded circular DNA of a geminivirus isolated from Digitaria sanguinalis has been sequenced. The data obtained are consistent with there being one DNA circle of 2701 nucleotides. Comparison of the nucleotide sewuence with those of maize streak virus (MSV) and wheat dwarf virus showed 64 and 47% DNA homology, respectively. The sequence has four potential coding regions for proteins of >10 kDa, two in the viral (+) sense and two in the complementary (−) sense. Each of these potential coding regions has a highly homologous counterpart among the seven open reading frames previously described for MSV. Virion DNA contained, in addition to the circular single-stranded DNA, a population of small DNA molecules similar to those associated with MSV particles. A comparison with MSV DNA of the region complementary to these small DNA molecules revealed conserved sequences, which may have a role in defining the limits of these primer-like molecules.