Journal of neurochemistry, 1984-03, Vol.42 (3), p.775-780
1984
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Details
- Autor(en) / Beteiligte
- Titel
- Partial Purification and Properties of the Inhibitors of Na,K‐ATPase and Ouabain‐Binding in Bovine Central Nervous System
- Ist Teil von
- Journal of neurochemistry, 1984-03, Vol.42 (3), p.775-780
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1984
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- : Endogenous inhibitors of Na,K‐ATPase and ouabain‐binding were partially purified from bovine central nervous system, and some of their properties were studied. They were eluted as low‐molecular‐weight fractions by gel filtration. They could be adsorbed by both Amberlite IR 120 and Amberlite IRA 400 at acidic and basic pH, respectively, indicating that they could act as both anions and cations at different pH. These inhibitors of ouabain‐binding appeared to affect specific binding of ouabin, and Scatchard plot analysis showed that the in hibition was competitive, suggesting that they could bind to the same site as ouabain, presumably to Na,K‐ATPase itself. The inhibitory activities were heat stable, but charring inactivated them completely.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-3042eISSN: 1471-4159DOI: 10.1111/j.1471-4159.1984.tb02749.xTitel-ID: cdi_proquest_miscellaneous_80935272
- Format
- –
- Schlagworte
- adenosinetriphosphatase, adenosinetriphosphatase inhibitor, Analytical, structural and metabolic biochemistry, Animals, Biological and medical sciences, Cattle, central nervous system, Chromatography, Gel, Chromatography, Ion Exchange, Endogenous inhibitors, Enzymes and enzyme inhibitors, Fundamental and applied biological sciences. Psychology, Hydrolases, Hypothalamus - enzymology, Kinetics, Na,K‐ATPase, Ouabain - metabolism, Ouabain‐binding, oubain, Protein Binding, Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors