In Vitro, 1983-12, Vol.19 (12), p.875-880
1983
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Details
- Autor(en) / Beteiligte
- Titel
- Growth and Morphology of Neuronal Cell Lines Cultured in Perfusion
- Ist Teil von
- In Vitro, 1983-12, Vol.19 (12), p.875-880
- Ort / Verlag
- Rockville, MD: Tissue Culture Association, Inc
- Erscheinungsjahr
- 1983
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- To optimize culture conditions and gain a more reliable culturing system for studies of metabolic properties of neuronal cells, a simplified perfusion chamber was developed. It consists of two parts: a perfusion block and a standard plastic culture dish. To confirm the suitability of this chamber for continuous culturing of anchorage-dependent cells, the growth and morphology of the four neuronal cell lines, glioma C6 and glioma 138MG, neuroblastoma C1300, clones N1E115 and N18 were followed for 4 d using both traditional and perfusion techniques. A marked increase in growth and a decrease in the degree of morphological differentiation were obtained with the latter technique compared to the former.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0073-5655eISSN: 2327-4328, 1475-2689DOI: 10.1007/BF02661707Titel-ID: cdi_proquest_miscellaneous_80849283
- Format
- –
- Schlagworte
- Animal cells, Animals, Biological and medical sciences, Biotechnology, Cell culture techniques, Cell cultures. Hybridization. Fusion, Cell Division, Cell growth, Cell Line, Cell lines, Cells, Cultured, Culture Media, Cultured cells, Establishment of new cell lines, improvement of cultural methods, mass cultures, Eukaryotic cell cultures, Fundamental and applied biological sciences. Psychology, Glioma, Glioma - pathology, Histological Techniques - instrumentation, Methods. Procedures. Technologies, Molecular and cellular biology, Neuroblastoma, Neuroblastoma - pathology, Neurons, Neurons - cytology, Perfusion, Tissue culture techniques, Ungulates