Details

Autor(en) / Beteiligte

• D’Afonseca, Vívian
• Prosdocimi, Francisco
• Dorella, Fernanda A.
• Pacheco, Luis Gustavo C.
• Moraes, Pablo M.
• Pena, Izabela
• Ortega, Josè Miguel
• Teixeira, Santuza
• Oliveira, Sérgio Costa
• Coser, Elisângela Monteiro
• Oliveira, Luciana Márcia
• Corrêa de Oliveira, Guilherme
• Meyer, Roberto
• Miyoshi, Anderson
• Azevedo, Vasco

Titel

Survey of genome organization and gene content of Corynebacterium pseudotuberculosis

Ist Teil von

• Microbiological research, 2010-05, Vol.165 (4), p.312-320

Ort / Verlag

Germany: Elsevier GmbH

Erscheinungsjahr

2010

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Corynebacterium pseudotuberculosis is an intracellular pathogen that causes Caseous lymphadenitis (CLA) disease in sheep and goats. The widespread occurrence and the economic importance of this pathogen have prompted investigation of its pathogenesis. We used a genomic library of C. pseudotuberculosis to generate 1440 genomic survey sequences (GSSs); these were analyzed in silico with bioinformatics tools, using public databases for comparative analyses. We employed non-redundant unique sequences as a query for BLAST searches against the genome, the translated genome and the proteome of four other Corynebacterium species that have been completely sequenced. We were able to characterize approximately 8% of the genome of C. pseudotuberculosis, including previously undescribed functional group genes, based on the COG database; the GSSs classification into categories gave 13% information storage and processing, 14% cellular processes and 23% metabolism. We found a close relation between C. pseudotuberculosis and C. diphtheriae conserved-gene synteny in Corynebacteria species.